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51.
The dependence of the acidolysis reaction of a C6-C3 dimeric nonphenolic β-O-4 type lignin model compound, 2-(2-methoxyphenoxy)-1-(3,4-dimethoxyphenyl) propane-1,3-diol (veratrylglycerol-β-guaiacyl ether, VG), on the type of acid applied was examined using three different acids [0.2 mol/l HCl, 0.2 mol/l HBr,
and 0.1 mol/l (0.2 N) H2SO4 in 82% aqueous 1,4-dioxane at 85°C]. In the HCl system, the major reaction modes of the corresponding benzyl cation-type
intermediate (BC), which is produced by protonation of the α-hydroxyl group of VG and successive release of the water molecule, are the abstraction of the β-proton and hydride transfer from the β-to the α-position. The liberation of formaldehyde from the γ-hydroxymethyl group of BC is the predominant reaction mode in the H2SO4 system. Apparently, an unknown reaction mode or modes is operative in the early stage of the HBr system that causes rapid
disappearance of VG accompanied by the quantitative formation of 2-methoxyphenol without affording the common counterpart
of a Hibbert’s ketone, 1-hydroxy-3-(3,4-dimethoxyphenyl) propan-2-one. The reaction mode in the HBr system changes with the
progress of the reaction and is the same as that in the HCl system after the early stage. 相似文献
52.
To improve the properties of particleboard, boards were produced using a sealed press. With the sealed press, boards were
processed under high-temperature and high-pressure steam. This increased the saturation temperature, causing a dramatic rise
in temperature inside the board, faster curing of the binder, and a shorter pressing time. The boards were bonded with urea
formaldehyde resin, melamine urea formaldehyde resin, or poly(methylene diphenyl diisocyanate) (PMDI). The sealed press improved
the internal bond strength and thickness swelling of boards regardless of the binder used during the reduced pressing time.
The increased bonding strength improved the board properties, allowing PMDI with a lower resin content to be used for bonding
the boards. 相似文献
53.
Naoki Harada Masaya Nishiyama Shigeto Otsuka Satoshi Matsumoto 《Soil Science and Plant Nutrition》2005,51(3):361-367
Phototrophic purple bacteria (PPB) are one group of dinitrogen (N2 )-fixing bacteria often found in the anoxic and photic zone of paddy soil. In the present study, the effects of inoculation of PPB on grain yield of rice and changes in their populations and nitrogenase activity were investigated with and without surface application of rice straw in a pot experiment. As an inoculant, Rhodopseudomonas palustris strain KN122 isolated from a paddy soil was selected, and the cell suspension was inoculated into the floodwater once or three times during the cultivation. As a result, the inoculation of PPB increased the grain yield of rice. Compared to the control, the grain yield was 9% higher in the inoculated plot without rice straw application. The treatment was more effective in combination with rice straw application. In the plots where PPB were inoculated with rice straw, grain yields were 21% (single inoculation) and 29% (triple inoculation) higher. Populations of PPB in the plots without rice straw application significantly increased by the inoculation, unlike those in the plots with rice straw application, except for the bulk soil. Phototrophic and heterotrophic nitrogenase activities (acetylene-reducing activity) associated with the soils or residues of the rice straw were not affected by PPB inoculation throughout the experiment. This indicates that the inoculation of PPB into floodwater may not be effective for enhancing N2 fixation in paddy soils and that the beneficial effect of PPB inoculation on the grain yield of rice may be due to unidentified functions of PPB other than biological N2 fixation. 相似文献
54.
Nobuhito Ohte Myron J. Mitchell Hideaki Shibata Naoko Tokuchi Hiroto Toda Goro Iwatsubo 《Water, air, and soil pollution》2001,130(1-4):649-654
To analyze the differences in the status and processes of nitrogen saturation in Japan and northeastern United States, we examined the hydrobiogeochemistry of nitrogen of forested watersheds in these regions. Two distinct differences were found between watersheds in Japan compared with those in US. 1) In Japanese watersheds, marked decreases of NO3 ? concentration in surface waters during the summer growing season were not found and NO3 ? concentrations sometimes increased especially in the summer at nitrogen saturated sites. This contrast with watersheds in US where decreases in NO3 ? concentration during the summer are commonly observed except those watersheds in advanced stages of nitrogen saturation. These differences in NO3 ? concentration relationships can be attributed to climatic differences, with Japan having high precipitation and high discharge during the summer, while in many regions of North America lowest discharges are found in the summer. The climatic regime in Japan leads to high rates of mineralization and the rapid transport of NO3 ? to streams in summer. 2) Japanese watersheds, even those with high NO3 ? concentrations in surface waters, show little evidence of acidification. This is in contrast to sites in US where increased NO3 ? concentrations, especially during episodic events, result in surface water acidification. 相似文献
55.
I. Noguchi T. Kato M. Akiyama H. Otsuka Y. Matsumoto 《Water, air, and soil pollution》1995,85(4):2357-2362
The precipitation chemistry in northern Japan, especially Hokkaido, has been investigated since 1982. This area has often been found to have high concentrations of alkaline road dust (asphalt dust) in the air, caused by the use of studded tires during the winter. It is well known that the composition of precipitation in these areas is often dominated by asphalt dust including calcium bicarbonate. However, recently the concentration of asphalt dust in the air has decreased owing to a ban on the use of studded tires. Simultaneously, in precipitation, the lowering of pH values and the increase of hydrogen ion depositions have been occurring owing to the decrease of non-sea-salt calcium ions (nss-Ca2+) concentrations and depositions derived from asphalt dust. In addition, we found that a decrease of nss-Ca2+ firstly leads to a decrease of bicarbonate ions (HCO3 ?), the counter ion to nss-Ca2+ in asphalt dust. Therefore, the increase of H+ concentrations and depositions was great in comparison with the decrease of nss-Ca2+ concentrations and depositions in areas where the HCO3 ?concentrations, varied by pH, and depositions had been low. Furthermore, this variation was mainly observed in the ionic composition of snow cover and snowfall at sites along the Japan Sea in northern Japan during winter. In this area, the Acid Shock effect may become a serious problem from the decline of pH values in melting snow. Moreover, we found that ammonium ions and non-sea-salt sulfate ions depositions have also been decreasing in response to a decrease of nss-Ca2+ depositions, derived from asphalt dust. It seems that this phenomenon is caused by the decrease of asphalt dust concentrations in the air. 相似文献
56.
In Caenorhabditis elegans, the gonad acquires two U-shaped arms by the directed migration of its distal tip cells (DTCs) along the body wall basement membranes. Correct migration of DTCs requires the mig-17 gene, which encodes a member of the metalloprotease-disintegrin protein family. The MIG-17 protein is secreted from muscle cells of the body wall and localizes in the basement membranes of gonad. This localization is dependent on the disintegrin-like domain of MIG-17 and its catalytic activity. These results suggest that the MIG-17 metalloprotease directs migration of DTCs by remodeling the basement membrane. 相似文献
57.
A 49-kilodalton phosphoprotein in the Drosophila photoreceptor is an arrestin homolog 总被引:10,自引:0,他引:10
T Yamada Y Takeuchi N Komori H Kobayashi Y Sakai Y Hotta H Matsumoto 《Science (New York, N.Y.)》1990,248(4954):483-486
The gene encoding the 49-kilodalton protein that undergoes light-induced phosphorylation in the Drosophila photoreceptor has been isolated and characterized. The encoded protein has 401 amino acid residues and a molecular mass of 44,972 daltons, and it shares approximately 42 percent amino acid sequence identity with arrestin (S-antigen), which has been proposed to quench the light-induced cascade of guanosine 3',5'-monophosphate hydrolysis in vertebrate photoreceptors. Unlike the 49-kilodalton protein, however, arrestin, which appears to bind to phosphorylated rhodopsin, has not itself been reported to undergo phosphorylation. In vitro, Ca2+ was the only agent found that would stimulate the phosphorylation of the 49-kilodalton protein. The phosphorylation of this arrestin-like protein in vivo may therefore be triggered by a Ca2+ signal that is likely to be regulated by light-activated phosphoinositide-specific phospholipase C. 相似文献
58.
Terracciano A Abdel-Khalek AM Adám N Adamovová L Ahn CK Ahn HN Alansari BM Alcalay L Allik J Angleitner A Avia MD Ayearst LE Barbaranelli C Beer A Borg-Cunen MA Bratko D Brunner-Sciarra M Budzinski L Camart N Dahourou D De Fruyt F de Lima MP del Pilar GE Diener E Falzon R Fernando K Ficková E Fischer R Flores-Mendoza C Ghayur MA Gülgöz S Hagberg B Halberstadt J Halim MS Hrebícková M Humrichouse J Jensen HH Jocic DD Jónsson FH Khoury B Klinkosz W Knezević G Lauri MA Leibovich N Martin TA 《Science (New York, N.Y.)》2005,310(5745):96-100
Most people hold beliefs about personality characteristics typical of members of their own and others' cultures. These perceptions of national character may be generalizations from personal experience, stereotypes with a "kernel of truth," or inaccurate stereotypes. We obtained national character ratings of 3989 people from 49 cultures and compared them with the average personality scores of culture members assessed by observer ratings and self-reports. National character ratings were reliable but did not converge with assessed traits. Perceptions of national character thus appear to be unfounded stereotypes that may serve the function of maintaining a national identity. 相似文献
59.
Excess cyclin E-Cdk2 accelerates entry into S phase of the cell cycle and promotes polyploidy, which may contribute to genomic instability in cancer cells. We identified 20 amino acids in cyclin E as a centrosomal localization signal (CLS) essential for both centrosomal targeting and promoting DNA synthesis. Expressed wild-type, but not mutant, CLS peptides localized on the centrosome, prevented endogenous cyclin E and cyclin A from localizing to the centrosome, and inhibited DNA synthesis. Ectopic cyclin E localized to the centrosome and accelerated S phase entry even with mutations that abolish Cdk2 binding, but not with a mutation in the CLS. These results suggest that cyclin E has a modular centrosomal-targeting domain essential for promoting S phase entry in a Cdk2-independent manner. 相似文献
60.
The observation of the regulation of fast protein dynamics in a cellular context requires the development of reliable technologies. Here, a signal regulation cascade reliant on the stimulus-dependent acceleration of the bidirectional flow of mitogen-activated protein kinase (extracellular signal-regulated kinase) across the nuclear envelope was visualized by reversible protein highlighting. Light-induced conversion between the bright and dark states of a monomeric fluorescent protein engineered from a novel coral protein was employed. Because of its photochromic properties, the protein could be highlighted, erased, and highlighted again in a nondestructive manner, allowing direct observation of regulated fast nucleocytoplasmic shuttling of key signaling molecules. 相似文献